DIGICARM Colección :
http://hdl.handle.net/20.500.11914/982
2024-03-28T11:19:41ZSilkworm gut fiber of "Bombyx mori" as an implantable and biocompatible light-diffusing fiber
http://hdl.handle.net/20.500.11914/2663
Título : Silkworm gut fiber of "Bombyx mori" as an implantable and biocompatible light-diffusing fiber
Autor : Cenis Anadón, José Luis; Aznar Cervantes, Salvador; Lozano Pérez, Antonio Abel; Rojo, Marta; Muñoz, Juan; Meseguer Olmo, Luis; Arenas, Aurelio
Resumen : This work describes a new approach to the delivery of light in deeper tissues, through a silk filament that is implantable, biocompatible, and biodegradable. In the present work, silkworm gut fibers (SGFs) of Bombyx mori L., are made by stretching the silk glands. Morphological, structural, and optical properties of the fibers have been characterized and the stimulatory effect of red laser light diffused from the fiber was assayed in fibroblast cultures. SGFs are formed by silk fibroin (SF) mainly in a β-sheet conformation, a stable and non-soluble state in water or biological fluids. The fibers showed a high degree of transparency to visible and infrared radiation. Using a red laser (λ = 650 nm) as source, the light was efficiently diffused along the fiber wall, promoting a significant increment in the cell metabolism 5 h after the irradiation. SGFs have shown their excellent properties as light-diffusing optical fibers with a stimulatory effect on cells.2016-07-01T00:00:00ZRearing diet may determine fish restocking success: a case study of hatchery-reared juvenile meagre, "Argyrosomus regius"
http://hdl.handle.net/20.500.11914/2643
Título : Rearing diet may determine fish restocking success: a case study of hatchery-reared juvenile meagre, "Argyrosomus regius"
Autor : Gil, María del Mar; Palmer, Miguel; Hernández Llorente, María Dolores; Gran, Amalia; Durán, Juana; García García, Benjamín; Jover, Miguel; Pastor, Elena
Resumen : [EN]
The resilience of released hatchery-reared specimens increases with age and size, but production costs are also greater for these individuals. Therefore, for a given budget, the consequences of increasing age and size impose a trade-off between producing a large number of vulnerable (small) fish or a small number of resilient (large) fish. Once the optimal size for releasing fish has been defined, the choice of rearing protocol will determine the number and quality of the fish that can be released. In this study, different rearing protocols were compared using meagre juveniles (Argyrosomus regius), which are presently the target of a restocking programme conducted in the Balearic Islands (western Mediterranean). Seven dif¬ferent diets were compared during the grow-out phase to identify the diets that produced good-quality juveniles of a given size at the lowest cost. Most of the diets produced juveniles of suitable biological quality in terms of growth, fish condition (relationships between length and total weight, liver weight and mesenteric fat weight) and tissue biochemical composi¬tion. A semi-moist diet (Diet G) provided the best growth rate, closely followed by commercial meagre pellets (Diet A). In contrast, the cost of growing fish with Diet A was lower for any possible size at release. This study demonstrates the need to consider both growth rate and production cost to select the rearing protocol that maximizes the number of juveniles that can be produced for a given budget and desired release size. These considerations will ultimately increase the chance of success of restocking programmes.; [ES]
La resiliencia de los ejemplares criados en cautividad y liberados se incrementa con la edad y el tamaño, pero los costes de producción también aumentan. Por lo tanto, para un presupuesto dado, estas consecuencias del incremento de la edad y el tamaño imponen un compromiso entre la producción de un gran número de peces vulnerables (pequeños) o un pequeño número de peces resistentes (grandes y de más edad). Una vez que el tamaño óptimo para la liberación de peces se ha definido, la elección del protocolo de cría determinará el número y la calidad de los peces que se pueden liberar con un presupuesto dado. En este estudio, se comparó la aplicación de diferentes protocolos de cría a juveniles de corvina (Argyro¬somus regius), que es la especie objetivo de un programa de repoblación llevado a cabo en las Islas Baleares (Mediterráneo occidental). Se compararon siete dietas diferentes durante la fase de engorde para identificar cuáles de ellas podrían producir juveniles de buena calidad, de un tamaño dado y al menor coste. La mayoría de las dietas produjeron juveniles de calidad bio¬lógica adecuada en términos de crecimiento, condición del pez (estimada a partir de la relación de la longitud con el peso to¬tal, el peso del hígado y el peso de la grasa mesentérica) y composición bioquímica del tejido. Una dieta semi-húmeda (Dieta G) proporcionó la mejor tasa de crecimiento, seguida de cerca por un pienso comercial de corvina (Dieta A). Por el contrario, el coste del cultivo de peces con la Dieta A fue menor para cualquier posible tamaño de suelta. Este estudio demuestra la ne¬cesidad de considerar tanto la tasa de crecimiento como los costes de producción, con el fin de seleccionar el protocolo de cría que maximiza el número de juveniles que pueden ser producidos para un determinado presupuesto y tamaño de liberación deseado. Estas consideraciones aumentarán, en última instancia, las posibilidades de éxito de los programas de repoblación.2015-12-01T00:00:00ZNurse's A-phase material enhance adhesion, growth and differentiation of human bone marrow-derived stromal mesenchymal stem cells
http://hdl.handle.net/20.500.11914/2585
Título : Nurse's A-phase material enhance adhesion, growth and differentiation of human bone marrow-derived stromal mesenchymal stem cells
Autor : Rabadán Ros, Rubén; Aznar Cervantes, Salvador; Mazón, Patricia; Ros Tárraga, Patricia; Aza, Piedad N. de; Meseguer Olmo, Luis
Resumen : The purpose of this study was to evaluate the bioactivity and cell response of a
well-characterized Nurse’s A-phase (7CaO_P2O5_2SiO2) ceramic and its effect compared to a
control (tissue culture polystyrene-TCPS) on the adhesion, viability, proliferation, and osteogenic differentiation of ahMSCs in vitro. Cell proliferation (Alamar Blue Assay), Alizarin Red-S (AR-s) staining, alkaline phosphatase (ALP) activity, osteocalcin (OCN), and collagen I (Col I) were evaluated. Also, field emission scanning electron microscopy (FESEM) images were acquired in order to visualise the cells and the topography of the material. The proliferation of cells growing in a direct contact with the material was slower at early stages of the study because of the new environmental conditions. However, the entire surface was colonized after 28 days of culture in growth médium (GM). Osteoblastic differentiation markers were significantly enhanced in cells growing on Nurse’s A phase ceramic and cultured with osteogenic medium (OM), probably due to the role of silica to stimulate the differentiation of ahMSCs. Moreover, calcium nodules were formed under the influence of ceramic material. Therefore, it is predicted that Nurse’s A-phase ceramic would present high biocompatibility and osteoinductive properties and would be a good candidate to be used as a biomaterial for bone tissue engineering.2017-04-01T00:00:00ZQuality and fruit colour change in Verna lemon
http://hdl.handle.net/20.500.11914/2584
Título : Quality and fruit colour change in Verna lemon
Autor : Porras Castillo, Ignacio; Brotons, J.M.; Conesa, A.; Castañer, R.; Pérez Tornero, Olaya; Manera, F.J.
Resumen : While most lemon cultivars in the northern hemisphere are harves¬ted in autumn-winter, Verna, an autochthonous Spanish cultivar, is harvested later (February to August), supplying the European market when lemons are in short supply, a market that is also served by imports from the southern hemisphere, mainly Argentina and South Africa. The aim of this study was to determine the temperature at which degreening begins naturally in Verna lemon, noting the evo¬lution of colorimetric parameter a and comparing the same with the equivalent measurements made in Eureka lemon, the most widely cultivated lemon worldwide. The influence of solar radiation on the colorimetric parameters in Verna was studied, and the influence of the minimum temperatures on the change from green to yellow, using the data collected over five growing seasons, was assessed. The results confirmed the relation between net solar radiation and degreening, a process that begins when net solar radiation reaches a value of between 2 and 4 MJ/m2.day and when the mean temperature of the 14 days prior to sampling is 8.8 °C or when the daily mean temperature reaches 5.5 °C on two consecutive days. The informa¬tion obtained will enable growers to predict the colour changes that will occur in the field and potential growers to ascertain whether a given geographical zone is suitable for the crop in question.2015-01-01T00:00:00Z